The one or two difference could have been from when wehave put the rubber tubing on to the conical flask. Discussion Catalase: Liver Substrate: Hydrogen Peroxide Looking at our data for the first experiment, we can infer that the liver contains the catalase enzyme while the hydrogen peroxide is the substrate. Place 2ml of the 3% hydrogen peroxide solution into a clean test tube. You will be using chicken or beef liver. Catalase breaks down hydrogen peroxide, which is toxic, into 2 safe substances- water and oxygen, by speeding up a reaction. Under which conditions does the enzyme work well, and under which ones does it work poorly? You have hundreds of different enzymes in each of your cells. Observations and results When exposed to hydrogen peroxide, did the blended liver bubble less when mixed with either the vinegar or baking soda compared with when it was untreated? You might think that they would die.
You will be examining some ofthe major factors, which influence the activity of an enzyme calledcatalase. The hypothesis is that after adding hydrogen peroxide to the liver, the reaction will begin and after heating it the reaction will quicken because heat often excelerates the rate of a reaction. Knowing that enzymes act as catalysts within a chemical reaction, the extent of color change present should shed light into the character of the given enzyme. As you add each test substance, record the reaction rate 0-5 for each tube. Systematic Errors The ruler we were using was quite old and dirty, with some of the finer millimeter markings rubbed off or obscured, leading to readings that were not as accurate as they could have been. Control variable 2: Amount of Hydrogen Peroxide.
The apple sample is simply carbohydrates meant as food for the apple seed and will have no enzymes in it, thus no reaction was observed. This enzyme, like many others, aids in the decomposition of one substance into another. Also when the reaction was very rigorous, the oxygen bubbles sometimes lifted the liver out of the hydrogen peroxide, causing it to stop reacting with the hydrogen peroxide. Place 2 ml of 3% hydrogen peroxide solution into a clean test tube and add a small piece of liver. Put one test tubes of liver and one of H2O2 into an ice bath. After testing each substance the group then began to look into optimal conditions of pH for the reactions.
Why do you think this is so? Put equal quantities of liver into 2 clean test tubes and 1 ml H 2O 2 into 2 other test tubes. The critical increment of induced inactivation is low in dilute hydrogen peroxide solutions but increases to a value of 30,000 calories in concentrated solutions of peroxide. Materials andMethods: At your lab table, youwill find everything you need to conduct today's experiments. We examined this through the use of Hydrogen Peroxide in its reaction with the catalase. Put equal quantities of liver into 2 clean test tubes and 1 ml H 2 O 2 into 2 other test tubes. Generally large proteins, enzymes are made up of several hundred amino acids, and often contain a non-proteinaceuos group essential in the actual catalyst.
We measured the reaction by how many bubbles were produced and the rate at which the bubbles were produced. The data supports my hypothesis because the enzymes found in liver were able to decompose hydrogen peroxide into hydrogen and oxygen at a reasonably fast rate. Rotate the chamber 180 degrees on its side so that the hydrogen peroxide solution comes into contact with the catalase-soaked disks. Hole punch four filter paper discs, pick them up with forceps, dip it into the stock catalase solution for a few seconds, and drain the disc against the sidewall of the beaker before you transfer it to the reaction chamber. After dropping the Hydrogen Peroxide on the apple samples there was no reaction indicated therefore receiving a rating of 0.
In the third experiment, it was inferred that as the acidity increased in the substrate, the rate of reaction decreased. The last part of the lab we experimented with different temperatures on the catalase to see if it would affect the reaction. What is this liquid composed of? Protocol Work as a team of 4 at your table to perform theexperiment. Changes in temperature, pH and concentration will have an effect on enzyme activity by speeding up or slowing down the. Enzymes are also sensitive to pH most enzymes perform optimally between a pH of 6-8, pH breaks ionic and hydrogen bonds denaturing the enzyme. Position the disks in the front bottomhalf of the reaction vessel the half nearest the opening.
The theory that catalase can be destroyed was tested in a lab setting using the procedures provided by Mrs. Key concepts Chemistry Acids Bases Temperature Physiology Molecular biology Introduction Your liver is important for cleaning up any potentially dangerous substances you consume. Which potato sample decomposed the most hydrogen peroxide? Add another 2ml of hydrogen peroxide to the liver remaining in the first test tube. Therefore the result would be 2, 4, 5, 7, 10, 11. The liver that was at 25°C had a huge amount of bubbles a 4 on the scale and the 0°C still had some bubbles but not as much a 2 on the scale. A water bath that hasbeen warmed to 33C.
Obtain a small amount of catalase solution in a 50 mL beaker. The critical increment of the catalytic decomposition of hydrogen peroxide by catalase is of the order 3000 calories. Which condition s makes it work the worst? Principle: The recommend rationale of the volume of enzyme lipase to sodium carbonate to substrate milk is 1:7:5. Therefore our hypothesis is somewhat correct. If we use potato or other tissue containing this enzyme, we can use this to measure the relative influence of varying several different factors on the activity of enzymes in living tissue. Figure 1 shows a pictureof the setup.
As you add each test substance, record the reaction rate 0-5 for each tube. Pour off the liquid into a second test tube. We will keep it same by using the same concentration of Hydrogen peroxide each experiment. What gas was produced by the breakdown of hydrogen peroxide? Hypothesis I think that when we test the surface area the bigger the surface area the quicker the reaction will be. Be careful not to splashH 2O 2 on the disks.
Check with your lab instructor for updatedinformation about which sections your group is responsible for. A possible source of error could be the scale at which we measured the reaction rate. The decomposition of hydrogen peroxide by catalase is regarded as involving two reactions, namely, the catalytic decomposition of hydrogen peroxide, which is a maximum at the optimum pH 6. Claim: Evidence: Reasoning: Lactaid is a product designed to help people who cannot digest milk sugar lactose because they are missing the enzyme lactase. Therefore, we know that the rate and the extent of.