Discuss factors that affect enzyme activity. What Environmental Factors Can Enzyme Activity Be Negatively Affected By? 2019-02-06

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Discuss the factors affecting the rate of enzyme activity

discuss factors that affect enzyme activity

When the substrate concentration is not limiting, the rate of an enzyme catalyzed reaction is directly proportional to the enzyme concentration. The velocity of the reaction is directly proportional to the amount of enzymes present, under constant conditions of temperature, pH and substrate concentration, i. Each enzyme operates within an pH range optimal pH. . These are known as inhibitor molecules. Rather, a very high concentration of enzymes where all the substrate molecules are already used up does not have any impact on the reaction rate.

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Factors that Affect Enzyme Activity

discuss factors that affect enzyme activity

However, that does not mean that a constant rise in concentration of enzymes will lead to a steady rise in the rate of reaction. As with the chemical catalysts, enzymes do not alter the equilibrium constant of the reaction they catalyze. The complex then forms the product P and releases the free enzyme: -In order to determine the catalytic activity of the enzyme, the decrease in substrate concentration or the increase in product concentration as a function of time can be measured. Also with an increase in substrate concentration the greater the collision frequency of substrate to enzyme active site. In the activation pathway, the transition state has the maximum energy.

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Discuss the factors affecting the rate of enzyme action.

discuss factors that affect enzyme activity

This is because the charge of its component amino acids changes with the change in the pH value. Activity declines both above and below the optimum pH. Effect of Activators : Some of the enzymes require certain inorganic metallic cations like Mg 2+, Mn 2+, Zn 2+, Ca 2+, Co 2+, Cu 2+, Na +, K + etc. Some of these reactions may not occur if the right kind of enzyme is not present in the body. When this occur enzyme losses it shape and the substrate is unable to bind properly to the active site thus reducing enzyme activity. The rate of the reaction is directly proportional to the quantity of enzymes available for it. Enzyme inhibitors interfere with the enzyme functions in two different ways.

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Factors Affecting Enzyme Activity: 6 Factors

discuss factors that affect enzyme activity

When the inhibitor binds to the enzymes it changes the shape of the enzyme thereby reducing the affinity of the substrate to the enzyme active site. Similarly, some bind to the enzyme molecule and consequently increase the reaction rate. These substances bind to the enzyme or the enzyme-substrate complex, thereby, affecting the rate. Its formation has a lower activation energy than the reaction between reactants without a catalyst. Vmax is reduced and Km remains the same since the substrate can still bind to the active site as well as before the inhibitor is present. Enzyme Concentration: Usually a very small amount of the enzyme can consume large amount of the substrate.

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Discuss the factors affecting the rate of enzyme activity

discuss factors that affect enzyme activity

This is called the activation energy. Substances which lower the reaction rate are called enzyme inhibitors and substances which increase the rate are as enzyme activators. Several factors affect the rate of an enzyme catalyzed reaction. This allows the protection of sensitive nutrients and aroma substances in foods. Many enzymes consist of a protein and a non-protein called the cofactor. This too blocks real reactions. The difference in free energy between transition state and ground state reactants is called the activation energy.

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Factors Affecting the Enzyme Activity

discuss factors that affect enzyme activity

You will be surprised to know that studies have found that it can make a chemical reaction 10 billion times faster. Effect of Substrate Concentration At a fixed enzyme concentration, an increase in f substrate concentration increases the rate of an enzyme § catalyzed reaction until the enzyme concentration becomes the limiting factor. To be precise, once the rate of reaction has attained stability, an increase in the quantity of enzymes does not affect the rate of reaction anymore. However, there are some specific enzymes which work well only in acidic or basic surroundings. Temperature: Like most chemical reactions, the rate of an enzyme catalyzed reaction increases as the temperature is increased. Variation in the reaction temperature, as small as 1 -2° C, may introduce a 10-20% increase in the reaction rate.

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FACTORS AFFECTING ENZYME ACTIVITY

discuss factors that affect enzyme activity

In the steady state, the intermediary complex concentration remains constant while the concentration of the substrate and end product are changing. The highest rate of reaction, known as the Initial Reaction Rate is the maximum reaction rate for an enzyme in an experimental situation. C The reaction rate decreases in this region in spite of an excess of substrate. In fact, this property of enzyme is made use in determining the activities of serum enzymes for diagnosis of diseases. The six factors are: 1 Concentration of Enzyme 2 Concentration of Substrate 3 Effect of Temperature 4 Effect of pH 5 Effect of Product Concentration and 6 Effect of Activators. But they do not undergo permanent changes and so remain unchanged at the end of the reaction.


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Factors affecting Enzyme Activity

discuss factors that affect enzyme activity

Find out more by looking at: For two molecules to react they must collide with one another. Because the enzymes are proteins, they will become denatured and turn inactive at the higher temperatures. Inhibitors and activators: Many molecules affect the rates of enzyme catalyzed reactions. These chemicals are called inhibitors, because they inhibit reaction. An enzyme lowers the activation energy by forming an enzyme- substrate complex and thereby increasing the reaction rate.


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What are the Factors that Affect the Enzymatic Activity?

discuss factors that affect enzyme activity

Effect of Temperature: Velocity of an enzyme reaction increases with increase in temperature up to a maximum and then declines. Enzyme Activity Explained An enzyme can increase the speed of a chemical reaction manifold. A simplified picture Route A reactant 1 + reactant 2 product Route B reactant 1 + enzyme intermediate intermediate + reactant 2 product + enzyme So the enzyme is used to form a reaction intermediate, but when this reacts with another reactant the enzyme reforms. They can only alter the rate of reaction, not the position of the equilibrium. This type of inhibitor reduces the ability of the enzyme to convert substrate into product. As the pH distances from the optimum, however, the reaction rate decreases because the shape of the enzyme's active site begins to deform, until it becomes denatured and the substrate can no longer fit the active site. When the rate of an enzymatic reaction is maximum and the enzyme is at its most active state, an increase in the concentration of substrate will not make any difference in the enzyme activity.

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